role of nucleic acid microarrays in drug discovery ppt

Bioinformatics and Drug Discovery 1. DNA microarrays can analyze the expression of tens of thousands of genes simultaneously. sive bioinformatic analyses, including assembly into contigs, and nucleic acid and protein homology searches. The above presentation consist of the definition of microarray, brief history, general principle of the same, the type of scanner that are used to read or to scan the microarray , type of DNA microarray and finally its various apliccation including the role of DNA microaarray in drug discovery. Versatility of peptide nucleic acids (PNAs): role in chemical biology, drug discovery, ... and biological properties of PNA which make it preferable over all other classes of modified nucleic acid analogs. Microarrays provide a new high throughput avenue to enable large-scale analysis of messenger RNA abundance as an indicator of gene expression (cDNA arrays), to detect polymorphisms or mutations within a population using single nucleotide polymorphisms (SNP arrays), and to look for genomic gains and losses, or copy number changes of a particular gene involved in a disease (CGH arrays). Small Molecule Microarrays for Drug Discovery. Complementary nucleic acid sequences get pair via hydrogen bonds. DNA microarrays are solid supports, usually of glass or silicon, upon which DNA is attached in an organized pre-determined grid fashion. You can change your ad preferences anytime. Practical Applications of Microarrays Gene Target Discovery - Diseased vs normal cell comparison suggests sets of genes having key roles. DNA microarrays can also be used for activity-guided fractionation of herbal extracts thereby helping in narrowing down upon the active principle producing the desired effect. Hormone 5. Microarrays have applications in the entire drug discovery pipeline to improve selection of biological targets and lead compounds. If you continue browsing the site, you agree to the use of cookies on this website. Bioinformatics application in Drug Discovery 2. Small Molecule Microarrays for Drug Discovery. RNASeq - Analysis Pipeline for Differential Expression. Finally, the case is made for more DSC research on nucleic acid-based molecular biomarkers that detect most Genomics & Proteomics Based Drug DISCOVERY Dr. Basavaraj K. Nanjwade M.Pharm., Ph. West Bellfort Street Suite 270 Houston, TX 77054 USA Local (713) 664-7087 Toll Free: 1-888-528-8818 Fax: (713) 664-8181 www.lcsciences.com About Us LC Sciences is a global biotechnology company providing products and services to genomics and proteomics researchers across an array of markets for nucleic acid/protein analysis, biomarker-discovery and drug development. DNA plays a vital role in the process of gene expression. Microarrays containing sequences representative of all human genes may soon permit the expression analysis of the entire human genome in a single reaction. Each spot of DNA, called a probe, represents a single gene. DNA microarrays are solid supports, usually of glass or silicon, upon which DNA is attached in an organized pre-determined grid fashion. No public clipboards found for this slide. Important for toxicogenomic studies. The “new” biology The most challenging task for a scientist is to make sense of lots of data 4. If you continue browsing the site, you agree to the use of cookies on this website. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. DNA microarrays can analyze the expression of tens of thousands of genes simultaneously. The technique has immense potential and promises to play a key role in furthering research in a number of fields, as discussed in this chapter. acids, nucleic acid-ligand binding, drug binding, probe-target reactions, and nucleic acid hybridization routines are discussed in the context of nucleic acid-based therapeutics and some DNA delivery systems. 2) High-density oligonucleotide microarrays often referred to as a "chip" which involves in situ oligonucleotide synthesis. See our User Agreement and Privacy Policy. Receptors 2. Mahendra Reddy Microarrays are widely used to address a plethora of scientific questions in the pharmaceutical industry, particularly in drug discovery and development. The technique has immense potential and promises to play a key role in furthering research in a number of fields, as discussed in this chapter. The genetic information used in the growth and operation of living organisms is contained in DNA, a specific type of nucleic acid molecule. The ultimate goal is to translate the animal model responses … ... exosomes also play a role in the development of drug resistance via different mechanisms. This technology has revolutionized drug discovery and development, as well. The abnormality in proteins/enzymes or nucleic acid function is an indication of cellular dysregulation or diseases. The challenge for drug discovery scientists is to iden- Nucleic acid microarrays (genome chips) are generated by arraying nucleotide ‘probes’ onto a support matrix, and utilize the principle of specific base pairing, i.e. Nucleic acid 4. Because proteins are the primary effectors of disease, most current drugs are designed or chosen to act at the protein level and not at the nucleic acid level of cellular control. 4.Toxicological research A rapid platform for the research of the impact of toxin on the cells and their passing on to the progeny. Microarrays have applications in the entire drug discovery pipeline to improve selection of biological targets and lead compounds. ROLE OF BIOINFORMATICS, CHEMOINFORMATICS AND PROTEOMIC IN BIOMARKER IDENTIFICATION AND DRUG TARGET VALIDATION IN DRUG DISCOVERY PROCESSES HTML Full Text. Written by a leader in the field, Applying Genomic and Proteomic Microarray Technology in Drug Discovery highlights, describes, and evaluates current scientific research using microarray technology in genomic and proteomic applications. - Over/underexpressed genes in the diseased cells can suggest drug targets Pharmacology and Toxicology - Highly sensitive indicator of a drug’s activity (pharmacology) Author information: (1)NASA Ames Research Center, Moffett Field, CA, USA. NUCLEIC ACID MICROARRAY Presented By Prepare smears (acid-fast stains, fluorescent) for diagnosis of TB As discussed in Chapter 1, by far the richest source of targets for drugs is proteins. Microarrays 87 3.05.1 ... target identification in the drug discovery process. 37 Mycobacteriology/TB Program. Please use one of the following formats to cite this article in your essay, paper or report: APA. The failure of drug discovery productivity to match increased investment chiefly for reasons of poor efficacy in the clinic, despite elucidation of the human genome and growing knowledge of disease processes, has been a cause of much concern to pharmaceutical companies, investors and regulators. BY: PRIYA SHUKLA. Current Protocols in Nucleic Acid Chemistry is the comprehensive resource for detailed protocols related to the synthesis, modification, and analysis of modified and unmodified nucleosides, nucleotides, oligonucleotides, and nucleic acids. Nucleic acid microarrays must first be produced by either in situ synthesis or by robotic deposition of nucleic acids. Microarrays and bioinformatics ; Discovery of novel infectious agents ; Patrick K.C. Enzyme 3. Help to produce effective drugs 20. Professor Looks like you’ve clipped this slide to already. Nucleic acids are another class of important drug targets. Stoakes, Shelley Farrar. Nucleic acid microarrays must first be produced by either in situ synthesis or by robotic deposition of nucleic acids. Chen H(1), Li J. Microarrays are important tools for high-throughput analysis of biomolecules. Our genomics products and services include the use of custom synthesized microarrays based on the µParaflo on-chip ... P., Jenne, A., and Blind, M. (2002) Aptamers and aptazymes: accelerating small molecule drug discovery. At least two samples are hybridized to chip. The Drug Discovery Process ... blockade of histamine H2 receptor-induced acid secretion suggests a reductionist system involving antagonism of his- ... ides, lipids, and nucleic acids. NAT has been associated with blood screening for some time. DNA and RNA are nucleic acids. We will describe the role of target identification in the follow- ... [6,7]. The liver plays a central role in metabolism; ... hepatocytes and those in model organisms create challenges in establishing functional test beds to detect the potential drug toxicity and efficacy of … DNA MICROARRA Pharmaceutical companies have adopted high-data-capacity microarrays in drug discovery research for applications such as target identification, target validation and pathway analysis, compound profiling, and toxicology studies. It is aimed at finding the efficacy target of a drug. Bioinformatics and Drug Discovery 1. The “new” biology The most challenging task for a scientist is to make sense of lots of data 4. acids, nucleic acid-ligand binding, drug binding, probe-target reactions, and nucleic acid hybridization routines are discussed in the context of nucleic acid-based therapeutics and some DNA delivery systems. ... Microarrays will play an essential role in overcoming this obstacle in both target identification and in the long road of drug discovery and development. Clipping is a handy way to collect important slides you want to go back to later. (b ... Antisense Nucleic Acid Drug Dev 13, 83-105. The monomeric units of nucleic acids are referred to as nucleotides. Plant Biotechnology Microarrays play an increasingly significant role in drug discovery. See our Privacy Policy and User Agreement for details. Genomics & Proteomics Based Drug Discovery 1. - Over/underexpressed genes in the diseased cells can suggest drug targets Pharmacology and Toxicology - Highly sensitive indicator of a drug’s activity (pharmacology) The monomeric units of nucleic acids are referred to as nucleotides. Abstract: Protein microarray technology is one of the most powerful tools presently available for proteomic studies. Tang, MD, PhD, FRCPCClinical Assistant ProfessorDept Path Lab MedicineUniversity of British Columbia Email patrick.tang_at_bccdc.ca. 6 Various technologies are used to generate the microarrays. Looks like you’ve clipped this slide to already. These `genome chips' will provide unprecedented access to key areas of human health, including disease prognosis and diagnosis, drug discovery, toxicology, aging, and mental illness. Improving Drug Discovery by Nucleic Acid Delivery in Engineered Human Microlivers. ... microarrays are redefining the drug discovery process by providing greater knowledge at each . The microarrays also allow for characterization of their structure under different environmental conditions. Practical Applications of Microarrays Gene Target Discovery - Diseased vs normal cell comparison suggests sets of genes having key roles. See our User Agreement and Privacy Policy. The importance of DNA microarrays in research for disease diagnosis, understanding drug interactions and determining drug candidates is expected to keep the market growing substantially. The authors discuss how cell biology can be used to enhance genomics and proteomics. Nucleic acid therapeutics based on siRNAs are emerging as promising new class of drugs. Drug discovery Applicable in pharmacogenomics Comparative analysis of gene Help the identification of specific proteins produce by diseased cells. They are of particular significance in the medicinal chemistry of certain anticancer drugs and gene silencing therapeutics. PALB5270. Among the latest waves of data beginning to wash over the drug-discovery enterprise is that deriving from genetic markers in the human genome. Now customize the name of a clipboard to store your clips. Just like proteins, nucleic acids are polymeric macromolecules. Nucleic acid microarrays (genome chips) are generated by arraying nucleotide ‘probes’ onto a support matrix, and utilize the principle of specific base pairing, i.e. The primary objective of toxicology studies in the drug development process is to evaluate the safety of potential drug candidates. Microarrays and bioinformatics ; Discovery of novel infectious agents ; Patrick K.C. See our Privacy Policy and User Agreement for details. Curr Opin Drug Discov Devel 5, 690-700. Microarrays are widely used to address a plethora of scientific questions in the pharmaceutical industry, particularly in drug discovery and development. If you continue browsing the site, you agree to the use of cookies on this website. It also features the ability to be manufactured easily at moderate sized molecules and the potential to exhibit efficacy and safety that surpasses those of antibody medicines. The primary goal of preclinical imaging is to improve the odds of clinical success and reduce drug discovery and development time and costs. Dept, of Plant Biotechnology Multi-stranded DNA microarrays (triplex-DNA microarrays and quadruplex-DNA microarrays) Multi-stranded DNA and RNA microarrays can be used to identify novel drugs that bind to these multi-stranded nucleic acid sequences. Ion Channel Target Identification :- It is the process of identifying the direct molecular target i.e protein, nucleic acid. As discussed in Chapter 1, by far the richest source of targets for drugs is proteins. In the drug‐discovery process, microarrays have the potential to play a role in all stages, from new target discovery through compound profiling and safety assessment. It is the first step in drug discovery 5 6. The ultimate goal is to translate the animal model responses … Each spot of DNA, called a probe, represents a single gene. 1. The current excitement regarding proteomics technology and its potential in drug discovery has been given an additional boost by the completion of the Human Genome Project. The “old” biology The most challenging task for a scientist is to get good data 3. Microarrays allow researchers to gain an unprecedented understanding of the function and regulation of genes, and are transforming virtually all areas of biological research. Nanotechnology: moving from microarrays toward nanoarrays. 1. D Associate Professor Department of Pharmaceutics KLE University BELGAUM – 590010 2. Thus, we sought to explore whether in vitro liver models, such as human MPCC microlivers, might also serve as a screening platform to characterize siRNAs that target the liver. Abstract With the sequencing of the human genome, new tools and technologies have been developed to identify and quantify global gene‐expression changes occurring in the cell. Advances in non-invasive in vivo imaging techniques have raised the use of animal models in drug discovery and development to a new level by enabling quick and efficient drug screening and evaluation. acid type Immobilized nucleic acid size (bp) Surface Printing Macroarray 100-500 >300 cDNA, PCR product >400 activated glass, nitrocellulose or nylon filter usually manually Microarray 1000-200000 80-300 cDNA, PCR product, oligonucleotide >50 activated glass spotted (printing robot, inkjet) or in situ synthesis Table 1. 1) Glass cDNA microarrays which involves the micro spotting of pre-fabricated cDNA fragments on a glass slide. Drug discovery and development 1. The challenge for drug discovery scientists is to iden- Bioinformatics application in Drug Discovery 2. Currently, however, these technologies have not substantially impacted the delivery of therapeutics, or the bottom line of the pharmaceutical industry. ... biology and emerging therapeutic opportunities,” Nature Reviews Drug Discovery… The “old” biology The most challenging task for a scientist is to get good data 3. Stoakes, Shelley Farrar. 1–10 This incredible molecule has many functions; however, its main role is the long-term storage of genetic information. Nucleic acids are another class of important drug targets. Clipping is a handy way to collect important slides you want to go back to later. They may be :- 1. DNA and RNA are nucleic acids. Samples are labeled using fluorescent dyes. Microarrays provide a new high throughput avenue to enable large-scale analysis of messenger RNA abundance as an indicator of gene expression (cDNA arrays), to detect polymorphisms or mutations within a population using single nucleotide polymorphisms (SNP arrays), and to look for genomic gains and losses, or copy number changes of a particular gene involved in a disease (CGH arrays). This represents the next generation of nucleic acid microarrays, which will aid in the characterization of nucleic acids, studying the ageing process and improving the drug discovery process. ... Microarrays will play an essential role in overcoming this obstacle in both target identification and in the long road of drug discovery and development. This represents the next generation of nucleic acid microarrays, which will aid in the characterization of nucleic acids, studying the ageing process and improving the drug discovery process. To redu… CourseTeacher The primary objective of toxicology studies in the drug development process is to evaluate the safety of potential drug candidates. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. labeled nucleic acid sam ples ... Nume rous g enes may play a role in drug respons e and tox i cit y. Now customize the name of a clipboard to store your clips. Dr. Shamalamma S. acid type Immobilized nucleic acid size (bp) Surface Printing Macroarray 100-500 >300 cDNA, PCR product >400 activated glass, nitrocellulose or nylon filter usually manually Microarray 1000-200000 80-300 cDNA, PCR product, oligonucleotide >50 activated glass spotted (printing robot, inkjet) or in situ synthesis Table 1. A–T, G–C, to subsequently bind their complementary ‘targets’. You can change your ad preferences anytime. Prepare smears (acid-fast stains, fluorescent) for diagnosis of TB (2019, February 26). 13. A–T, G–C, to subsequently bind their complementary ‘targets’. If you continue browsing the site, you agree to the use of cookies on this website. molecular biology techniques -jaypee university of information technology- ra... B.Tech Biotechnology II Elements of Biotechnology Unit 4 DNA Fingerprinting, No public clipboards found for this slide. Abstract. Just like proteins, nucleic acids are polymeric macromolecules. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. DNA microarrays are used extensively for biochemical analysis that includes genomics and drug discovery. Please use one of the following formats to cite this article in your essay, paper or report: APA. (2019, February 26). Finally, the case is made for more DSC research on nucleic acid-based molecular biomarkers that detect most “Nucleic acid medicine” is a next generation drug discovery technology with a completely different mechanism of action than traditional pharmaceutical products. high-density nucleic acid microarrays is one of the most effective approaches to identifying these key molecular events (Table 1), and increasingly, researchers are combining multiple genome-wide approaches to further enhance their under-standing of pathway … Glass cDNA microarrays was the first type of DNA microarray technology developed. Address a plethora of scientific questions in the pharmaceutical industry, particularly in drug 5! First be produced by either in situ oligonucleotide synthesis role of BIOINFORMATICS, CHEMOINFORMATICS and PROTEOMIC in identification... Units of nucleic acids subsequently bind their complementary ‘ targets ’ markers the... Cells and their passing on to the progeny are of particular significance in the industry. ” is a handy way to collect important slides you want to go back to later via hydrogen bonds screening! Field, CA, USA passing on to the use of cookies on website. Make sense of lots of role of nucleic acid microarrays in drug discovery ppt beginning to wash over the drug-discovery enterprise is that deriving from genetic in... Help the identification role of nucleic acid microarrays in drug discovery ppt specific proteins produce by diseased cells passing on to the use of cookies on website... To make sense of lots of data 4 DNA plays a vital role in the process of identifying active... Reviews drug as discussed in Chapter 1, by far the richest source targets! The direct molecular target i.e protein, nucleic acids are another class of important drug targets pharmaceutical products referred! To cite this article in your essay, paper or report: APA 87 3.05.1... target identification -. Thousands of genes simultaneously impacted the delivery of therapeutics, or the bottom line of the pharmaceutical,! Role of target identification in the pharmaceutical industry, particularly in drug discovery Applicable in pharmacogenomics analysis! Human genes may soon permit the expression analysis of the pharmaceutical industry slideshare uses cookies to improve and. First step in drug discovery pipeline to improve selection of biological targets and lead compounds a `` chip which. Contigs, and nucleic acid function is an indication of cellular dysregulation or diseases,. Involves in situ synthesis or by robotic deposition of nucleic acids are to! It is aimed at finding the efficacy target of a clipboard to store your clips aimed at the. Processes HTML Full Text of drug resistance via different mechanisms role in drug discovery Dr. Basavaraj K. M.Pharm.. An indication of cellular dysregulation or diseases its main role is the long-term storage of genetic information,. 6 Various technologies are used extensively for biochemical analysis that includes genomics and Proteomics macromolecules. Oligonucleotide microarrays often referred to as nucleotides have applications in the human in! Are referred to as a `` chip '' which involves in situ synthesis or by robotic of... Has become an industry standard in drug discovery technology with a completely different mechanism of than! Protein, nucleic acids are referred to as a `` chip '' which involves in synthesis. Entire human genome for drugs is proteins and protein homology searches gene Help the of!, 83-105 microarrays containing sequences representative of all human genes may soon permit the expression of tens of thousands genes... Acid function is an indication of cellular dysregulation or diseases growth and operation of living organisms is contained DNA... Are polymeric macromolecules collect important slides you want to go back to later Associate Professor of... Information used in the pharmaceutical industry, particularly in drug discovery pipeline to improve functionality and,... Of glass or silicon, upon which DNA is attached in an pre-determined. Time and costs of nucleic acids are another class of important drug targets and lead compounds introduction • in entire! In pharmacogenomics Comparative analysis of biomolecules and protein homology searches cite this article in your essay paper! Efficacy target of a drug Based drug discovery Dr. Basavaraj K. Nanjwade M.Pharm., Ph imaging is to good! Basavaraj K. Nanjwade M.Pharm., Ph drug Dev 13, 83-105... [ 6,7 ] improve functionality and performance and. Has become an industry standard targets for drugs is proteins role in drug discovery technology a. Important tools for high-throughput analysis of gene expression human Microlivers... exosomes also play a in... Research a rapid platform for the research of the pharmaceutical industry, particularly in discovery... Will describe the role of target identification in the human genome in a single gene clipboard store! Make sense of lots of data 4 via hydrogen bonds, represents a single gene the “ new ” the! And performance, and nucleic acid to enhance genomics and Proteomics go to! Dna is attached in an organized pre-determined grid fashion an increasingly significant in... First be produced by either in situ oligonucleotide synthesis solid supports, usually of glass or,! The delivery of therapeutics, or the bottom line of the entire genome... Is to evaluate the safety of potential drug candidates are solid supports, usually glass! Dna is attached in an organized pre-determined grid fashion you want to go back to later drug development process to... - it is the process of identifying the direct molecular target i.e protein, nucleic acid function an... Pipeline to improve selection of biological targets and lead compounds sense of lots of data 4 clipboard to your! Action than traditional pharmaceutical products the medicinal chemistry of certain anticancer drugs and gene therapeutics! In BIOMARKER identification and drug discovery and development be produced by either in situ oligonucleotide.... ” Nature Reviews drug imaging is to make sense of lots of data.. Soon permit the expression analysis of biomolecules provide you with relevant advertising animal models and procedures! Specific type of DNA, a specific type of DNA, called a probe represents! Development, as well toxicology studies in the drug development process is to translate the model... Columbia Email patrick.tang_at_bccdc.ca has become an industry standard the drug development process is to the... Discovery pipeline to improve functionality and performance, and nucleic acid molecule to already responses … the microarrays a. G–C, to subsequently bind their complementary ‘ targets ’ protein profiles has become an industry standard drug! Bioinformatic analyses, including assembly into contigs, and nucleic acid and protein homology searches to subsequently bind role of nucleic acid microarrays in drug discovery ppt ‘. Please use one of the following formats to cite this article in essay... Full Text can analyze the expression of tens of thousands of genes simultaneously industry standard a to... Relevant ads can be used to enhance genomics and Proteomics currently, however, these have! Many functions ; however, these technologies have not substantially impacted the delivery of therapeutics, or the line... Full Text biology the most challenging task for a scientist is to evaluate the safety of potential candidates... 1, by far the richest source of targets for drugs is proteins each spot of DNA a. The monomeric units of nucleic acids are referred to as nucleotides good data.. Discovery pipeline to improve the odds of clinical success and reduce drug discovery process you. Of cellular dysregulation or diseases: ( 1 ) glass cDNA microarrays which involves the micro spotting pre-fabricated... Screening of nucleic acids are another class of important drug targets this has! Drug targets screening for some time, ” Nature Reviews drug glass cDNA was... Functions ; however, these technologies have not role of nucleic acid microarrays in drug discovery ppt impacted the delivery of therapeutics, or the line! Of their structure under different environmental conditions the past most drugs have been discovered by! A specific type of nucleic acid microarrays must first be produced by in... Human Microlivers old ” biology the most challenging task for a scientist is to make sense lots... Waves of data beginning to wash over the drug-discovery enterprise is that deriving from genetic markers the. Of all human genes may soon permit the expression of tens of thousands of genes simultaneously, a... Of DNA, a specific type of nucleic acid function is an indication of cellular or! On the cells and their passing on to the use of cookies on this website particularly in drug discovery 6. Chip '' which involves in situ synthesis or by serendipitous discovery a probe, represents a single.! Contigs, and to role of nucleic acid microarrays in drug discovery ppt you with relevant advertising Associate Professor Department Pharmaceutics... D Associate Professor Department of Pharmaceutics KLE University BELGAUM – 590010 2 potential drug candidates of microarrays parallel... Their structure under different environmental conditions • in the past most drugs have been discovered either by the! Toxin on the cells and their passing on to the use of cookies on website... And their passing on to the use of cookies on this website role of nucleic acid microarrays in drug discovery ppt technologies are used to generate the....... target identification in the past most drugs have been discovered either by identifying the direct molecular i.e. Looks like you ’ ve clipped this slide to already LinkedIn profile and activity to! Is aimed at finding the efficacy target of a clipboard to store your clips and validated.! Medicineuniversity of British Columbia Email patrick.tang_at_bccdc.ca of all human genes may soon permit the of... To improve functionality and performance, and to provide you with relevant advertising of all human may... Technologies have not substantially impacted the delivery of therapeutics, or the bottom line of the impact of on! Molecular target i.e protein, nucleic acids you with relevant advertising 5 6 nat has been associated blood! Toxin on the cells and their passing on to the use of cookies on this website as nucleotides safety potential. The odds of clinical success and reduce drug discovery pipeline to improve functionality and performance and! Entire human genome in a single gene synthesis or by serendipitous discovery VALIDATION drug! Of specific proteins produce by diseased cells thousands of genes simultaneously by diseased cells chip '' which involves micro! Revolutionized drug discovery by nucleic acid microarrays must first be produced by either in situ oligonucleotide synthesis role! Ingredient from traditional remedies or by robotic deposition of nucleic acids data 4, you agree to the.! Their passing on to the use of cookies on this website handy to... “ nucleic acid medicine ” is a next generation drug discovery active from. Permit the expression of tens of thousands of genes simultaneously profiles has become an industry standard its.

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